Abstract: It is an important step to identify the mine water source for preventing and controling water, so the selection of the appropriate method is the key to identify the source of water fast and high efficient. This paper briefly introduces the principle and steps of cluster analysis, and applies it in the mine water source identification. According to the mine water chemistry data, analyzing the water chemistry features, ensuring the indicators of water type determination, the paper establishes the model of water source identification which including the system clustering analysis. Finally, based on the existing water standard samples data, through imitating the water source determination through the simulation of MATLAB, the results proves the effect of identifying sources of water based on cluster analysis.