Abstract: According to the difficulties of complex business process, scattered experimental data and corresponding file storage, difficult to retrieve and process experimental data, low experimental data security in the gas disaster prevention laboratory, this study carried out demand analysis and business process combing, associated the experimental data of existing experimental projects with their corresponding sample information, designed functional modules and system architecture, realized basic data management, mandatory management, report management and comprehensive query functions, developed a laboratory information management system（ＬＩＭＳ） based on B/S structure. Compared with traditional manual management, LIMS provided a more convenient management method, improved data management capabilities and work efficiency of testing and inspection personnel, reduced laboratory operating costs and realized functions such as fast traceability and report output.