Abstract: In order to study the influence of safety sign attribute characteristics on visual attention of subjects under different light intensities, three attributes of frame color, frame shape and background contrast color were selected as independent variables. Under different light intensities, eye movement tracking technology was used to record the first fixation time and the number of fixation points of the subjects, and the differences of visual attention effects were compared. SPSS26.0 software was used for data analysis. The results showed that under different light intensities, the difference of safety sign attributes could significantly affect the visual attention of the subjects. Under high light intensity, the participants’ first fixation time on red, triangle and white safety signs was shorter, and the number of fixation points was more, and the visual attention effect was better. Under low light intensity, subjects paid more attention to green, triangle and white contrasting colors.