Abstract: In order to comprehensively grasp the development of roof karst and hydraulic connection in the second mining area of Qinglong Coal Mine in the northern Guizhou Mining Area, the designated area near Tuomeihe in the second mining area of Qinglong Coal Mine is taken as the main research object, and three methods of geological analysis, transient electromagnetic exploration and drilling verification are adopted to conduct analysis and research. The results show that there is a compound aquifer in the roof of No.16 coal in the second mining area of the mine, and the water content of each aquifer is not uniform. According to the results of data, several low-resistivity abnormal areas were divided and delineated, and five abnormal areas were found. Among them, the range of I and II abnormal areas was relatively large and the amplitude was relatively strong. The anomaly range of III, IV and V is relatively small and the amplitude is relatively medium. Comprehensive analysis shows that I and II abnormal areas are the main abnormal areas of No.16 coal seam roof at present. Through the field drilling verification, the verification result and the detection result have a high coincidence degree, and the application effect is good.