Abstract: A micro-seismic monitoring substation based on precision clock (PTP) synchronization technology was developed. This article introduces the software and hardware design of substation and the principle and implementation of precision clock synchronization protocol. The equivalent input noise and time synchronization accuracy of the substation are tested. The conclusion shows that the maximum equivalent input noise of the substation is 10.04 μV; the synchronization accuracy of the substation and the time server is 320 ns; the synchronization between the substation and the substation is 120 ns. The positioning error of the underground fixed-point blasting test of the KJ768 micro-seismic monitoring system based on this substation is less than 10 m, which is convenient for on-site installation and meets the needs for monitoring of rock burst mines.