Abstract: In order to study the influence of methane-oxidizing bacteria on the industrial properties of coal sample, we prepared modified coal samples with different concentrations of methane-oxidizing bacteria liquid and compared coal samples. The methane-oxidizing bacteria were cultivated in the soil, and the bacterial solutions with concentrations of 100%, 75% and 50% were prepared. The samples with the same size and quality were poured into the coal samples. The industrial properties of modified coal samples with different concentrations of methane bacteria were analyzed by industrial analyzer, and the volatile content, ash content and fixed carbon content of coal samples before and after the action of methane-oxidizing bacteria were compared. The results showed that the volatile content of modified coal samples treated with different concentrations of bacterial liquid increased and the fixed carbon content decreased to some extent. For the bacteria solution of GY group, with the increase of the concentration of bacteria s