Abstract: With the annual increase of raw coal output in China, safety production situation of coal mine is still severe. Coal mine accident has seriously affected the psychological state of miners and finally led to the change of physical signs. By establishing an emergency scene experiment system for coal mine accidents, the physical signs of the experimental subjects before and after the experiment were tested, and the significant physiological evaluation indexes were obtained to analyze the influence of gender and accident experience on the physiological indexes. Based on physiological reaction of the miners’ psychological quality, three suggestions are proposed to provide reference basis for the evaluation, psychological training and adjustment of key post personnel of coal enterprises.