模拟实体煤赋存环境下厌氧微生物降解煤吸附甲烷实验
Experiment on Degradation of Coal Adsorbed Methane by Anaerobic Microorganism Under Simulated Solid Coal Occurrence Environment
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摘要: 厌氧型甲烷氧化菌是一类以甲烷作为唯一碳源和能源的微生物，在降解转化煤层吸附瓦斯等方面具有广阔的应用前景。自主设计了原煤体钻孔菌液注入系统、厌氧微生物降解原煤体吸附甲烷实验分析系统。实验结果表明：30 ℃为该菌种最适宜生长温度，甲烷最高降解率可达47.73% ；在注液压力为0~22 MPa范围内，甲烷降解率随着注液压力的增大而增大，注液压力为22 MPa时，原煤体被压裂，此时甲烷降解率急剧增大，平均降解率达到63.79%；甲烷降解率随着注入菌液体积的增加而增大，注入菌液为35 mL时甲烷的降解率约为55%。Abstract: Anaerobic methane oxidizing bacteria is a kind of microorganism that can used as the sole carbon source and energy source of methane, which has broad application prospects in the degradation and conversion of coal absorbing gas. We designed the drilling fluid injection system of raw coal, degradation analysis experiment system of coal absorbing gas by anaerobic microorganism independently. The experimental results show that: 30 ℃ is the most suitable growth temperature of the anaerobic methane oxidizing bacteria, the highest degradation rate is up to 47.73%; in the range of 0 MPa to 22 MPa of injection pressure, the degradation rate of methane increased with the increasing of injection pressure, when the injection pressure is 22 MPa, the raw coal is fractured, the degradation rate increases sharply, the average degradation rate reaches to 63.79%; methane degradation rate increases with the increasing of the volume of the injected liquid, the degradation rate of methane is about 55% when the bacterial suspension is 35 mL.
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[1] 张子敏.瓦斯地质学[M].徐州:中国矿业大学出版社,2009:3-4. [2] 韩冰,苏涛,李信,等.甲烷氧化菌及甲烷单加氧酶的研究进展[J].生物工程学报,2008,24(9):1511. [3] 闵航,谭玉龙,吴伟祥,等.一个厌氧甲烷氧化菌菌株的分离、纯化和特征研究[J].浙江大学学报(农业与生命科学版),2002,28(6):619-624. [4] 陈中云,闵航,陈美慈,等.不同水稻土甲烷氧化菌和产甲烷菌数量与甲烷排放量之间相关性的研究[J].生态学报,2001,21(9):1498-1505. [5] 吴自军,周怀阳,彭晓彤.珠江口桂山岛沉积物甲烷厌氧氧化作用研究[J].自然科学进展,2007,17(7):905-912. [6] 尹希杰,陈坚,郭莹莹,等.九龙江河口沉积物中硫酸盐还原与甲烷厌氧氧化:同位素地球化学证据[J].海洋学报,2011,33(4):121-128. [7] 韩丹,石峰,柴晓利,等.生活垃圾填埋场甲烷自然减排的新途径:厌氧与好氧的共氧化作用[J].环境科学学报,2011,31(4):791-797. [8] 刘妍妍,龙焰,尹华.硝酸盐对矿化垃圾中兼/厌氧甲烷氧化的影响[J].环境科学,2013,34(11):4349-4355. [9] 沈李东,胡宝兰,郑平.甲烷厌氧氧化微生物的研究进展[J].土壤学报,2011,48(3):619-628. [10] Islas Lima S, Thalasso F, Hernandez G. Evidence of anoxic methane oxidation coupled to denitrification[J]. Water Research, 2004, 38(1): 13-16. [11] Anderson RT, Lovely DR. Naphthalene and benzene degradation under Fe(III) reducing conditions in petro-leum-contaminated aquifers. Bioremediation Journal, 1999, 3(2):121-135. [12] 张瑞林,崔学锋.厌氧微生物降解原煤体吸附甲烷试验研究[J].煤炭科学技术,2016,44(5):155-159. [13] 周蕾.厌氧烃降解产甲烷菌系的组成及其代谢产物的特征[D].上海:华东理工大学,2012:5-6.
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