Abstract: The inspection effect of deep hole fixed-point sampling experiment was carried out at the working face of 12033 coal seam in Zhongling Mine. According to the results of initial sampling, the sampling device was optimized. The results of numerical simulation and late experiment effect of sampling showed that sampling tail plait cascaded with 6-nozzle and 8-nozzle injector had the biggest negative pressure that could achieve -11 164 Pa, and the maximum sampling depth of the optimized sampling device achieved 102 m, and took out more coal samples and the sampling time was less than 5 min. The optimized sampling device applied to sampling requirements in Zhongling Mine and had higher popularization value.