Abstract: Single separation layer or multiple separation layer group often occurred by the deformation incoordination of the roadway surrounding rock. Based on the elastic analysis of a single separation layer, the bolt stress distribution caused by the multiple separation layer group was summarized according to the superposition principle. The multiple peaks curves of stress distribution was got. By means of the parameters analysis under different situations, the analysis found that the higher of roof separation layer value and the roof separation layer location occurred closer to the boundary，the higher of the additional stress occurred from the bolt by the roof separation layer would be. The influence of roof separation layer on shearing stress is bigger than axial force of anchorage body. Anchorage interface will produce sliding failure because of big roof separation layer value.